Part:BBa_K4296001
2A self-cleaving peptide (P2A)
2A self-cleaving peptides (the class to which P2A belongs) are peptides about 18-22 amino acids long, that when tagged to proteins of interest, trigger ribosomal skipping during translation. By adding P2A in between two protein coding sequences (e.g. proteinA-P2A-proteinB), all under the same promoter; during translation A and B can be translated as separate polypeptides. This is because the ribosomal skipping results in no peptide-bond being formed between the two different polypeptides (A and B) even though they come from the same mRNA sequence. So using P2A is essentially a way to generate multiple proteins simultaneously, without needing to place each protein under a separate transcriptional unit.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 37
- 1000COMPATIBLE WITH RFC[1000]
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